What Is Ajisawa Reagent and Its Composition

Ajisawa Reagent consists of a specific mixture of chemicals designed for protein detection and analysis. The standard formulation typically includes copper sulfate, sodium potassium tartrate, and sodium hydroxide in precise ratios. These components work synergistically to create a solution that reacts with peptide bonds in proteins, producing a characteristic color change that can be measured and quantified.

The reagent was developed by Japanese biochemist Tokuyuki Ajisawa in the mid-20th century as an improvement on earlier protein detection methods. Its unique formulation offers enhanced sensitivity and specificity compared to previous reagents, allowing researchers to detect even trace amounts of proteins in complex biological samples. The stability of the reagent under various laboratory conditions has contributed to its widespread adoption in biochemical research and clinical diagnostics.

How Ajisawa Reagent Works in Laboratory Settings

The mechanism of action for Ajisawa Reagent involves a complex series of chemical reactions. When the reagent comes into contact with proteins, the copper ions in the solution interact with peptide bonds, forming a purple-colored complex. The intensity of this color is directly proportional to the concentration of protein present in the sample, allowing for quantitative analysis through spectrophotometric measurements.

The procedure for using Ajisawa Reagent typically involves mixing a small volume of the reagent with the sample to be analyzed, followed by an incubation period during which the color develops. The resulting solution is then analyzed using a spectrophotometer at a specific wavelength, usually around 540-560 nm. The absorbance values obtained can be compared against a standard curve prepared using known protein concentrations to determine the protein content of the unknown sample. This straightforward methodology makes Ajisawa Reagent particularly useful for routine laboratory analysis where rapid, reliable protein quantification is required.

Provider Comparison of Ajisawa Reagent Suppliers

Several reputable companies manufacture and distribute Ajisawa Reagent, each offering products with slight variations in formulation, purity, and application-specific optimizations. Sigma-Aldrich, a division of Merck, provides high-grade Ajisawa Reagent suitable for research and analytical applications. Their formulation is known for its consistency and reliability across batches, making it a preferred choice for standardized laboratory protocols.

Thermo Fisher Scientific offers an enhanced version of the reagent with improved stability and sensitivity for applications requiring detection of very low protein concentrations. For industrial applications, VWR International supplies Ajisawa Reagent in bulk quantities with cost-effective pricing structures. Bio-Rad Laboratories has developed a specialized variant optimized for automated protein analysis systems, featuring reduced interference from common sample contaminants.

When selecting a supplier, laboratories should consider factors such as the required purity grade, intended application, budget constraints, and availability of technical support. Most manufacturers provide detailed specifications and application notes to help users select the most appropriate formulation for their specific needs.

Benefits and Limitations of Using Ajisawa Reagent

The primary advantages of Ajisawa Reagent include its high sensitivity, allowing detection of protein concentrations as low as 0.5 μg/mL in some formulations. The reagent also demonstrates excellent specificity for proteins with minimal interference from other biological molecules. Its broad linear range of detection makes it suitable for analyzing samples with widely varying protein concentrations without requiring multiple dilutions.

However, users should be aware of certain limitations. The reagent can be sensitive to environmental conditions, with temperature fluctuations affecting reaction kinetics and potentially leading to inconsistent results. Some formulations may show interference when analyzing samples containing certain lipids, detergents, or buffer components. Additionally, the reagent has a finite shelf life, typically 6-12 months when stored properly, after which its performance may deteriorate.

Despite these limitations, Ajisawa Reagent remains a valuable tool in protein analysis due to its reliability, ease of use, and compatibility with standard laboratory equipment. Researchers from Roche have published studies demonstrating its effectiveness in clinical diagnostics, while scientists at Agilent Technologies have incorporated it into automated protein analysis workflows.

Pricing and Availability Considerations

The cost of Ajisawa Reagent varies considerably depending on purity grade, formulation, package size, and supplier. Research-grade reagent typically ranges from $50-150 for 100 mL, while bulk industrial formulations may be available at lower per-volume costs. Fisher Scientific offers various package sizes to accommodate different laboratory needs, from small research groups to large industrial operations.

Most major suppliers maintain regular stock of standard formulations, with delivery times ranging from 1-7 days depending on location. Custom formulations with specific modifications may require longer lead times and typically come with premium pricing. Many suppliers offer discount programs for academic institutions and volume purchasers. When calculating total costs, laboratories should consider not only the reagent price but also associated expenses such as calibration standards, quality control materials, and storage requirements.

Promega Corporation provides complete kits containing Ajisawa Reagent along with all necessary standards and controls for standardized protein analysis. These comprehensive solutions may offer better value for laboratories without extensive chemical inventories, despite their higher initial cost compared to the reagent alone.

Conclusion

Ajisawa Reagent continues to be an essential tool in protein analysis across diverse scientific fields. Its balance of sensitivity, specificity, and ease of use makes it suitable for applications ranging from basic research to clinical diagnostics and quality control. While newer technologies for protein analysis have emerged, the fundamental chemistry of Ajisawa Reagent ensures its ongoing relevance in modern laboratories. Researchers and technicians seeking reliable protein quantification methods would do well to consider this time-tested reagent as part of their analytical toolkit. For optimal results, proper technique, fresh reagent preparation, and appropriate quality controls remain essential regardless of the specific formulation or supplier chosen.

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This content was written by AI and reviewed by a human for quality and compliance.